Biotin 抗体 (Texas Red (TR))
Quick Overview for Biotin 抗体 (Texas Red (TR)) (ABIN401199)
抗原
适用
宿主
克隆类型
标记
应用范围
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产品特性
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Absorption / Emission: 596 nm / 620 nm
Molar Ratio: 4.8 Moles Texas Red? per mole of Rabbit IgG -
纯化方法
- Affinity chromatography
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免疫原
- Biotin conjugated to Keyhole Limpet Hemocyanin (b-KLH)
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亚型
- IgG
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anti-Biotin antibody
WB, ELISA, IHC, FACS, IF, FM 宿主: 小鼠 Monoclonal 4C7-D5 unconjugated
anti-Biotin antibodyWB, IHC, FACS, IF, ISt, CM 宿主: 小鼠 Monoclonal Hyb-8 unconjugated
anti-Biotin antibodyIHC, ISt 宿主: 兔 Monoclonal BTN-2032R unconjugated Recombinant Antibody
anti-Biotin antibodyWB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Biotin antibody (DyLight 488)WB, FACS, IF, FM, FLISA, DB, MA 宿主: 山羊 Polyclonal DyLight 488
anti-Biotin antibody适用: 人 WB 宿主: 兔 Monoclonal unconjugated
anti-Biotin antibodyELISA, FACS 宿主: 小鼠 Monoclonal SB58c unconjugated
anti-Biotin antibodyELISA, FACS, IP, MS 宿主: 小鼠 Monoclonal 1D4-C5 unconjugated
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应用备注
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Immunofluorescence: 1/500 - 1/2,500. Flow Cytometry: 1/2,000 - 1/10,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- Restore with 1.0 mL of deionized water (or equivalent).
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浓度
- 1.0 mg/mL (by UV absorbance at 280 nm)
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缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containings 10 mg/mL BSA as stabilizer and 0.01 % (w/v) Sodium Azide as preservative
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- DO NOT FREEZE!!
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储存条件
- 4 °C
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储存方法
- Store the antibody undiluted at 2-8 °C.
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- Biotin
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物质类
- Chemical
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背景
- Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein's biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells. Rockland Immunochemicals produces anti-epitope tag antibodies against many common epitope tags including Myc, GST, GFP, 6X His, MBP, FLAG and HA. Rockland Immunochemicals also produces antibodies to other tags including FITC, Rhodamine (TRITC), DNP and biotin.
抗原
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